Effect of Different Colchicine Concentrations on Chromosome Polyploidy Behavior of Gall Oak (quercus Infectoria Oliv.) by Using Molecular Biology

Gall oak has a large ecological adaptability landscape and social importance it is share of the natural wealth great economic value. Polyploidy deducted in this study through pre-screened directly by determining stomata guard cell length then confirmed quantifying DNA content using SCoT-PCR analysis. Depending as method we found maximum tetraploids (4n) mixoploids (3n) plants were induced chlciploidy treatments 2000 3000 mgl-1 colchicine soaked for 96 h. 24 can detect polyploidy Oak provides an alternative analyzing polyploid genotype breeding. Ungerminated seeds increased with increasing concentrations exposure period especially colchiploidy (2000 72 h.) result toxicity substance. Significant differences observed between polyploids diploids plants. The tetraploid plants, show superiority phenotypical treaties created taller thicker seedlings stems roots, had more yields branch, leaves roots per seedlings, bigger leaves. Whereas, chemical physiological characteristics, significant contents chlorophyll which leads to greener leaf color, but decreased tannins amount. Also, cells size number area. Inducing novel features ploidy breeding powerful tool that lead commercial success forest tree species